Extraction and scavenging activities on free radicals in- vitro of a polysaccharide from Semen coicis

نویسندگان

  • Yijun Fan
  • Heqin Li
  • Aoxue Luo
چکیده

The water-soluble polysaccharide (SCP) was isolated from Semen Coicis through hot water extraction followed by ethanol precipitation. The polysaccharide was deproteinized by using the Sevag reagent, and the polysaccharide was free of proteins as scan by UV Spectra in 260 nm and 280 nm. In order to evaluate its antioxidant activities, Free radicals scavenging activities on superoxide radicals, hydroxyl radicals, DPPH and ABTS in vitro were carried out. The results indicated that SCP has significant radicals scavenging abilities on superoxide radicals and hydroxyl radicals and strong reducing capacity. The scavenging effects were powerful, which closed to the positive control. Therefore, the polysaccharide SCP should be explored as novel potential antioxidants.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Free radical scavenging activity of antitumour polysaccharide fractions isolated from Ganoderma lucidum (Fr.) P. Karst

The study was conducted to investigate the free radical scavenging activities of polysaccharide fractions extracted from the fruiting body of medicinal mushroom, Ganoderma lucidum (Fr.) P. Karst (Reishi) using different antioxidant capacity assays. Four intracellular polysaccharide fractions were obtained by the hot-water extraction and precipitation with ethanol, by ammonium oxalate extraction...

متن کامل

In vitro antioxidant effects of barberry fruit extracts

A vast majority of the studies addressing the free radicals including hydroxyl radical is a damage compound of biochemical molecules such as DNA, proteins and lipids. When free radicals specially hydroxyl radical are not adequately removed from the body, it may damage biological macromolecules, leading to a variety of disease occurs. Therefore, the body should be protected by an enzymatic or no...

متن کامل

Antioxidant capacity and radioprotective properties of the flavonoids galangin and kaempferide isolated from Alpinia galanga L. (Zingiberaceae) against radiation induced cellular DNA damage

Background: Alpinia galanga L. belonging to the family Zingiberaceae is widely grown in the state of Kerala, India. They are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. The efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic DNA was studied. Materials and Methods: The bioflavonoids, galangin and k...

متن کامل

Antioxidant activity of polysaccharide-enriched fractions extracted from pulp tissue of Litchi Chinensis sonn.

Natural antioxidants such as polysaccharides with strong antioxidant activities are used to protect against oxidative damage, yet little is known so far about the antioxidant effects of litchi fruit polysaccharides. In the present study, four different polysaccharide-enriched fractions were isolated from litchi pulp tissue and partially purified by a stepwise method of ethyl alcohol (EtOH) prec...

متن کامل

Rapid screening of antioxidant activity, fracture rate and scavenging of free radicals by hairy root of Periwinkle (Catharanthus roseus L. G. Don)

Background & Aim:Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. We aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rol...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015